| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-94
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-565
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6857
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate |
2012-08-22^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2017-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-101796689-B1 |
| titleOfInvention |
Screening and Engineering Method of Super-Stable Immunoglobulin Variable Domains and Their Uses |
| abstract |
The present invention provides a gene construct encoding a fusion protein in which a target protein and an antibiotic resistance protein are bound to a Tat signal sequence and expressing the construct in E. coli to produce a target protein that is soluble and has excellent thermodynamic stability, A method named Tat-Associated Protein Engineering (TAPE) which selects a target protein which is a cell-derived immunoglobulin variable domain (VH or VL) and a method named water-soluble protein selected by the TAPE method And for human VH and VL domain antibodies and human VH and VL domain antibody scaffolds with excellent thermodynamic stability. The present invention also relates to a library containing a random CDR sequence in the VH or VL domain antibody backbone selected by the TAPE method and a method for producing the same, VH or VL domain antibodies and therapeutic pharmaceutical compositions comprising such domain antibodies. |
| priorityDate |
2012-08-22^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |