http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101865870-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-327 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-26004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2011-05-25^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-11^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-11^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101865870-B1 |
| titleOfInvention | Modified RNAse H and detection of nucleic acid amplification |
| abstract | A 'hot start' RNAse H enzyme composition reversibly modified for the detection of an improved CATACLEAVE ™ probe of a nucleic acid sequence in a test sample is disclosed. An important feature of the enzyme composition is its ability to modulate the catalytic activity of RNAse H during the reverse-PCR reaction. Thus, RNAse H activity can initially be suppressed to minimize degradation of the RNA: DNA primer heteroduplex prior to reverse transcription. After the cDNA synthesis is complete, RNAse H activity is induced and CATACLEAVE < RTI ID = 0.0 > (TM) < / RTI > annealing to the target DNA sequence in the reverse transcriptase- It facilitates probe cutting and fluorescence detection. The inductive RNAse H enzyme can be used as a first step reverse transcriptase CATACLEAVE < RTI ID = 0.0 > It is suitable for high-speed bulk analysis requiring PCR. |
| priorityDate | 2010-05-25^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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