http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-19990030510-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-00 |
| filingDate | 1997-10-01^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1999-05-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-19990030510-A |
| titleOfInvention | Mass production method of antibiotics using redox-cycling reagent |
| abstract | The present invention relates to a method of mass-producing clavalonic acid in culture by activating the transcription of genes involved in the biosynthesis of clavalulanic acid by adding redox-cycling reagents to generate microorganisms during microbial culture. will be.n n n Streptomyces clavuligerus produces clavalonic acid (CA), a clinically important β-lactamase inhibitor. The production of this acid is increased by treatment with several selected redox-cycling reagents. Stimulating effects are seen with the addition of menadione (MD), plumbagin (PLM), and phenazine methosulfate (PMS), but negative effects with the addition of methyl biorogen (MV). PMS increases the accumulation of this acid by about 150% and the induction of superoxide dismutase (SOD) on PMS addition suggests that free radicals are involved in the process. The stimulatory effect of PMS is offset by the addition of butyrated hydroxyanisole (BHA), which supports the involvement of free radicals. The increased production of this acid by free radicals is due to the transcriptional activation of the gene cas2, which encodes the key enzyme, in CA biosynthesis.n n n In the present invention, the active oxygen was confirmed to increase the biosynthesis of several antibiotics in addition to CA as a secondary metabolite through transcriptional activation of the biosynthetic gene. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100646829-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015023049-A1 |
| priorityDate | 1997-10-01^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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