http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20000019366-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5ad8562c578b061df7a2c3aee9d0e24 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0004 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 1998-09-10^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e83f67c05fd980a6decab832102963cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d11b2a7d52dd3b943b8c53699c75d5ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14b321308c3e0393510e999c73778e6d |
| publicationDate | 2000-04-06^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20000019366-A |
| titleOfInvention | Novel Membrane Binding 2-Keto-Di-Gluconate Dehydrogenase Gene Sequence and 2,5-Diketo-Di-Gluconate Conversion Method |
| abstract | The present invention provides a cell sequence-binding 2-keto-D-gluconate dehydrogenase gene sequence isolated from Erwinia herbicola strain and the gene without intracellular metabolism. 2,5-diketo-di-gluconate was converted to 2-keto-di-gluconate by recombinant 2-keto-di-gluconate dehydrogenase by introducing into other strains. A method for converting to (2,5-diketo-D-gluconate) is disclosed. Cloning and subcloning of cell membrane-bound 2-keto-di-gluconate dehydrogenase gene from the above Erwinia herbiola ATCC 08111 strain. After determining the nucleotide sequence of the keto-di-gluconate dehydrogenase gene, the recombinant plasmid pKI111 inserted with the gene was introduced into E. coli, followed by transformation of E. coli and culturing. By expressing the membrane-bound 2-keto-di-gluconate dehydrogenase gene, 2-keto-di-gluconate was converted to 2,5-diketo- by recombinant cell membrane-bound 2-keto-di-gluconate dehydrogenase activity. Di-gluconate has an excellent effect of rapid conversion without intracellular metabolism. |
| priorityDate | 1998-09-10^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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