| abstract |
The present invention relates to a method of screening sex of sperm and artificially fertilizing the selected sperm through ICSI, and more specifically, through a FISH / CISH analysis using a probe that complementarily binds to the sex chromosome of sperm. Sex-selective artificial insemination via ICSI which selects sex and injects the selected sperm into the cytoplasm of the egg. The present invention also measures the survival rate of selected sperm using MTT reduction assay based on the enzyme action of mitochondria. In addition, single cell electrophoresis is used to measure the toxicity of the formazan produced, the treatment of the MTT material, and the damage to DNA that may occur using an absorbance system.n n n By using the FISH / CISH method of the present invention it is possible to overcome the efficiency and stability problems at the same time compared to the conventional sex screening method. In addition, the sperm selected through the FISH / CISH process can be induced to have a specific sex according to ICSI, and the sex of the sperm can be produced to produce a mammal having a specific sex. Therefore, the present invention may ultimately enable the mass production of pigs for long-term bio-production. |