| abstract |
The present invention relates to an expression vector of a recombinant protein using an RNA polymerase alpha subunit as a fusion partner, and a method for producing a recombinant protein using the same, and specifically, to an expression vector connecting genes of a fusion partner and a foreign protein. In the production method of recombinant protein comprising the steps of preparing, introducing the expression vector into a host cell to prepare a transformant, and culturing the transformant to induce the expression of the recombinant protein and obtain the same, RNA polymerization Method for producing a recombinant protein, characterized in that to improve the water solubility and folding (folding) of the recombinant protein by expressing the gene of the enzyme alpha subunit using the fusion partner and expression vector containing the gene of the RNA polymerase alpha subunit as the fusion partner It is about. The method for producing a recombinant protein using the gene of the RNA polymerase alpha subunit of the present invention as a fusion partner can overcome the limitations on water solubility and folding of the existing fusion partner, and is widely used for protein medicine and industrial production. Can be used. |