http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20180113469-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_050fc7b82c5d17d9aeb63a7cdcc546b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44f04d319aaa6e66f240244bc905f452 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P5-026 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P5-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-69 |
| filingDate | 2018-04-05^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a5e52d73129c78306d10e61b7f4e521 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f072f5f88f6b28c1adccc9a9a704bd5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bbb92796918a0a4a695309ff627f943 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fed353bb0e248e14436696b5d3c3d8ab |
| publicationDate | 2018-10-16^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20180113469-A |
| titleOfInvention | Modulation of plasmid copy number in antibiotics-free plasmid maintenance system |
| abstract | The present invention relates to a synthetic 5 'untranslated region (UTR), a gene expression cassette comprising a promoter and regulatory gene, a replication origin and a recombinant vector comprising said gene expression cassette, an improved segregational instability in which said recombinant vector is introduced A method for producing a recombinant microorganism having improved separation anxiety by introducing the recombinant vector, and a method for quantitatively controlling the number of plasmid copies in a recombinant microorganism. The gene encoding the translation initiation factor necessary for the cell according to the present invention, infA And Escherichia coli as a host are introduced into a gene expression cassette containing the regulatory gene to remove the efp encoding the protein elongation factor (EF-P) from the chromosome of the microorganism, and the plasmid can be isolated from the antibiotic- It can be stably maintained. Further, in the recombinant microorganism, infA And efp When the expression level is precisely controlled through a promoter, PCN can also be quantitatively controlled with high efficiency, and can be widely applied in various industries for producing recombinant proteins. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110804617-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110804617-B |
| priorityDate | 2017-04-06^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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