| abstract |
The present invention discloses monooxygenase mutants and their applications. The amino acid sequence of the monooxygenase mutant is an amino acid sequence obtained by mutating the amino acid sequence shown in SEQ ID NO: 1, and the mutation is site 49, site 60, site 61, site 144, site 145 , 146th site, 147th site, 167th site, 169th, 189th, 246th, 247th, 280th, 284th, 285th, 286th, 287th, th a mutation site of at least one of a 328 site, a 330 site, a 332 site, a 382 site, a 427 site, a 428 site, a 429 site, a 430 site, a 431 site, a 432 site, and the like; The monooxygenase mutant has the advantage that stereoselectivity is greatly improved, and the enzyme activity is also increased accordingly. |