http://rdf.ncbi.nlm.nih.gov/pubchem/patent/PL-156255-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-16 |
| filingDate | 1986-10-17^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1992-02-28^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | PL-156255-B1 |
| titleOfInvention | A method of producing expression vectors for transforming Yarrowia lipoiytica |
| abstract | The method of producing ckpression vectorsn57 ^ t to transform Yarrowia lipoiytica,ncharacterized in that the plasmid pXHP-24 cleavesnusing HindIII-Aval enzymes to obtain episode on1150 base pairs long, this segment is isolated andnligated against T4 ligase with a synthesis stretchntic DNA sequence:n5 'nCCGAGAITCCIGCTTCTTCTAAGTCCAAGCGAnj 'nJ 'nCTAAGGACGAAGAAGATTACGGTTCGCTTGAn5 'nthen the product obtainednthe ligation is cleavednHindIII-BamHI enzymes to produce a stretchnka with a length of 1196 base pairs, which is a segment afternis isolated, ligated in the presence of T4 z ligasen440 bp segment derived fromndigestion with BamHI-XmaI enzymes of the plasmid pLS-n3 and with a segment of approximately 8.0 kilobase pairsnderived from digestion with enzymes HindIII-nXm, a1 of the plasmid pLS-3 then obtainednE. coli is transformed with the product of the ligation reaction,na selection is made of transformants resistant tonampicillin and isolated from these transformantsnplasmid pXX-33. |
| priorityDate | 1985-10-18^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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