http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2249045-C2
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 1999-08-11^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2005-03-27^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a4a9e43bf15cd50d011dc03024fa286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db4ee4a21be27ea5b3923c7c2e703550 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4c027aa36094b9203d283a7600b633c |
publicationDate | 2005-03-27^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2249045-C2 |
titleOfInvention | Method for identifying and characterization of function potentially presenting in nucleic acids-containing biological sample |
abstract | FIELD: molecular biology, medicine, pharmaceutical industry. n SUBSTANCE: invention discloses a method for identifying of definite functions of transcripts and proteins encoding by nucleic acid containing in the sample, a set for its realization comprising specific plasmid vector. Indicated vector comprises ori region, gene encoding tRNA and a cloning site located between two strong promoters optionally flanked by terminators and wherein the additional selective gene can be incorporated. Nucleic acid fragments obtained from a sample containing nucleic acid are fused into vector and vector is treated in vitro to obtain transcripts. Indicated transcripts are used in processes for testing their functions or for preparing proteins encoding by them. Proteins functions are tested also. A set for carrying out the method comprises, respectively, cellular extract for translation, agent for polymerization, agents that are necessary for testing one or some functions of proteins or transcripts encoding their, buffers, agents that are necessary for preparing nucleic acid fragments and specific vector. Applying invention provides rapid and effective carrying out function associated with polynucleotide sequence comprising in biological sample that involves nucleic acids. n EFFECT: improved identifying method. n 27 cl, 7 dwg, 11 tbl |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2478709-C2 |
priorityDate | 1998-08-12^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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