http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2548719-C1

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_511f6422bd60c264b64e83866b0736ec
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
filingDate 2013-12-27^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-04-20^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d4fdcbdb94709538cdea477933e05dd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12018de9dfcbd31fbb7fa7b648df00cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c94a56963cbe59c88361ecc84c03b731
publicationDate 2015-04-20^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2548719-C1
titleOfInvention METHOD OF DIAGNOSING TOXIGENIC STRAINS Clostridium difficile
abstract FIELD: biotechnology. n SUBSTANCE: fecal sample of a patient is kept at 96% absolute alcohol for 30-60 min in the ratio of alcohol and feces of 1:1, centrifuged for 15-20 minutes, then the fecal sample is treated with 1% solution of sodium deoxycholate in a ratio of 1:1 for 30-60 minutes, centrifuged, the supernatant is removed, then the primary inoculation of precipitation in the medium with addition of 1% lactose and 0.5-1.0 mM arabinose is carried out, incubated in anaerobic culture apparatus for 24-48 hours, the grown colonies are applied to the standard discs impregnated with the chromogenic substrate - ortho nitrophenyl-β-D-galactosidase. When appearance of bright yellow colour of the disc the toxigenic strains of Clostridium difficile are diagnosed. n EFFECT: use of the claimed method enables to diagnose toxigenic strains of Clostridium difficile with high sensitivity and specificity, simplicity and accessibility. n 3 ex
priorityDate 2013-12-27^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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