http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2636346-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_23afbaf9885ffdfa029bf0f887ab502c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-21 |
| filingDate | 2016-07-01^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-22^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32a38784132f1155495a7971ad00823a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b5c997f477a2d8ec9f9d67dc19521ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5efe39dbcd0ed076d51c9ebc0c51b407 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60ec42ab34d780ccbc6bef644b6a3982 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52666f2d942fafb90dd6e146041ce066 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cac0d65b5343311b47f7e03629461d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37fae0833151e302215435d7acdd30ec |
| publicationDate | 2017-11-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2636346-C1 |
| titleOfInvention | Method for obtaining of recombinant exoprotein of a pseudomonas aeruginosa |
| abstract | FIELD: biotechnology. n SUBSTANCE: method includes obtaining of expression plasmid vector pET-rEPA (SEQ NO: 3), containing a promotor sequence of T7 bacteriophage DNA and DNA sequences encoding the N-end region of protein product of translation enhancer (MASMT amino-acid sequence), six histidine residues, site of SUMO protease cleavage and a sequence (SEQ NO: 1) optimized for broadcasting in E. coli encoding recombinant rEPA protein (SEQ NO: 2). Recombinant chimeric precursor protein in the heterological expression system in electro-competent cells of E. coli BL21 (DE3), transformed by the plasmid vector pET-rEPA to obtain a E. coli BL-rEPA strain at 37°C to ensure the maximum amount of accumulation of precursor protein in the soluble fraction. Lysis of the bacterial mass is carried out in the presence of 4% Triton X-100 to preserve the precursor protein in a soluble form. The precursor protein is isolated by metal chelate chromatography on Talon sorbent charged with Co 2+ ions, followed by hexahistidine and SUMO peptide cleavage with SUMO protease from the polypeptide. The cleaved recombinant protein rEPA is subject to finish purification by anion-exchange chromatography on DEAE-Sepharose sorbent and gel-filtration chromatography on a Superdex 200-filled column, with translation to the target buffer with pH of 7.5, obtaining the recombinant rEPA protein. n EFFECT: production of protein in high yield. n 4 dwg, 4 ex |
| priorityDate | 2016-07-01^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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