http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1543345-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76b91954946ed607d765ea5bb9ac1a76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 1987-04-28^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1990-02-15^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8386cefc51c0abed9a45de4f920e65eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135715654e8a700979812b2154978a68 |
| publicationDate | 1990-02-15^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SU-1543345-A1 |
| titleOfInvention | Method of determining degree of inflammatory process in chronic bronchitis |
| abstract | The invention relates to medicine, in particular to laboratory diagnostics, and can be used to determine the degree of inflammation activity in chronic bronchitis. The aim of the invention is to improve the accuracy of the method. To do this, patients with chronic bronchitis on an empty stomach take sputum, homogenize it, dilute 2 times with saline, add 1% solution of Triton X-100 in an amount equal to 1/10 of the sample volume, cool and centrifuge for 20 minutes at 5000 rpm In the supernatant, the cathepsin activity of their hemoglobin cleavage is determined. To do this, 1.0 ml of a solution containing 20 mg / ml of hemoglobin in acetate buffer, with a pH of 4.0 with the addition of 120 mg / ml of urea, is added to 0.1 ml of the supernatant. The test tube is incubated in a closed form for 1 h at 37 ° C, 2.0 ml of 4% aqueous solution of trichloroacetic acid are poured in, stirred and centrifuged at 3000 rpm for 15 minutes. The control from the experimental one is different in that in the control @ 0.1 ml of sputum supernatant is introduced into the tube after the addition of trichloroacetic acid. In samples, the optical density is determined at a wavelength of 280 nm and the activity of cathepsins is calculated by the formula A = E / k . C, where A is the activity of cathepsins in sputum, is expressed in nM tyrosine / h . mg of proteinn n n K - calibration coefficient, equal to 0.42n n n C - protein concentration in the sample, mgn n n E-units of extinction. For indicators A less than 130 nM tyrosine / h . mg protein set I degree, 130-260-II degree and with |
| priorityDate | 1987-04-28^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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