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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
filingDate 2018-10-31^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-09-15^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_427283d154c65ce02e8354a1d4b43746
publicationDate 2020-09-15^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10774368-B2
titleOfInvention Nucleic acid preservation solution and methods of manufacture and use
abstract Disclosed is nucleic acid preserving compositions and methods of manufacturing and using the same. Compositions include a carrier, a chaotropic agent, a buffering agent, a chelating agent, a surfactant, an alcohol, an acid, and a mucolytic agent. Compositions as aqueous solutions can include water as a carrier. Preferred embodiments include water, guanidine thiocyanate, Tris, EDTA, SLS, SDA 3C, HCl, and N-acetyl-L-cysteine. Some embodiments include a colored dye as a visual indicator. Methods of manufacturing include combining the components into a mixture, such as an aqueous solution. Methods of use include providing a biological sample that includes nucleic acid and contacting the biological sample with the composition. Kits include the composition disposed in a portion of a biological sample collection apparatus.
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