| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8f0d61d62d86c276f17f51948cdebc45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8adaddc602ad7cb7f9e2d550681bb36d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0636
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0877
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0487
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00587
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-513
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00389
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-537
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00286
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00353
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00418
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00529
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-507
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-141
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00675 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-50273
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502738
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-629
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-137
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-501
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
| filingDate |
2017-12-15^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-10-20^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_842d1a0d0b2ba38b71eb9b9d61d6ae61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4826ef2d71969a140e398fe7cc2d783d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1130ea64f0af545d8ca3d23c994c3576
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be48e77d15e4229ba1600d732ddf0af6 |
| publicationDate |
2020-10-20^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-10808277-B2 |
| titleOfInvention |
Kinetic exclusion amplification of nucleic acid libraries |
| abstract |
An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites. |
| priorityDate |
2017-01-05^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |