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filingDate 2015-11-20^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-11-24^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-10845305-B1
titleOfInvention Method for increasing sensor resolution by spectrally stacking responsive dyes
abstract An analyte-sensitive substance is provided that has an optical property related to the concentration of an analyte. The analyte-sensitive substance includes an ionophore or other substance configured to provide a local pH, within the analyte-sensitive substance, that is related to the concentration of the analyte proximate the analyte-sensitive substance. The analyte-sensitive substance further includes a pH-sensitive fluorophore that increases or decreases its intrinsic fluorescence intensity with the local pH across a specified range of pH values. The analyte-sensitive substance further includes a pH-sensitive quencher configured to increase the slope of the change of fluorescence intensity of the pH-sensitive fluorophore across the specified range of pH values. The analyte-sensitive substance may further include an ionic additive configured to adjust the local pH such that the specified range of pH values corresponds to a range of analyte concentration values of interest.
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