| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bda5416e591d3d33059f20c4f65f9c90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f8a360f588f8b213ac7279a1448d2830 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-531
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3513
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2522-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-121
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-151
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6811
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2017-12-07^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2023-01-17^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d26299f5ed8aa98107e5812ea0d1aa0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a33437e4e1c946c7e64d657dc77ee434
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45f8d4fa846db7f65f6c98d34f0746de
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4667621907f058dda4cb6b89c34e80f4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7798a14ea151408d64353bf4a00cb99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4303dbcb62ee35f8c83ffc103fac182c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c0e2d8c95b517680397ff0f0feee5d3 |
| publicationDate |
2023-01-17^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-11555187-B2 |
| titleOfInvention |
RNA-directed DNA cleavage by the Cas9-crRNA complex |
| abstract |
Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit. |
| priorityDate |
2012-03-20^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |