| abstract |
Improved biosensors are provided having excellent selectivity and stability properties, together with methods of preparing the biosensors. A preferred biosensor includes an electrode ( 12 ) having enzyme ( 16 ) deposited thereon together with a layer of electropolymerized polymer ( 18 ) intermingled with the enzyme ( 16 ); a crosslinked silane film ( 20 ) is applied over the polymer layer ( 18 ), and a final coating ( 22 ) of polyurethane is formed over the film ( 20 ). In preparative procedures, the enzyme ( 16 ) is electrodeposited using an aqueous enzyme solution containing a nonionic surfactant at a concentration level preferably in excess of the critical micelle concentration of the surfactant. In the case of a glucose sensor, the polymer layer ( 18 ) is preferably polyphenol, while the silane film is crosslinked (3-aminopropyl) trimethoxysilane. The preferred biosensors have greatly enhanced selectivity stabilities. |