| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate |
2002-10-11^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_532aad28635832abf63bb7d0102d25ab |
| publicationDate |
2003-09-18^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003175749-A1 |
| titleOfInvention |
Annealing control primer and its uses |
| abstract |
The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010036108-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1856280-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1856280-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013288246-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017260576-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3252168-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015045258-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8198027-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023034913-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008305482-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010514420-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10619198-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8211899-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10415092-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10407723-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9677135-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008527979-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8642268-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010184159-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109576291-A |
| priorityDate |
2001-12-08^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |