| abstract |
The metabolic processes involved in the formation of any glucose-based metabolite are determined. A precursor molecule is labeled with a stable carbon ( 13 C) isotope at specific positions. The label is allowed to distribute and rearrange in the system. Metabolites are recovered and analyzed against a control system to determine metabolic pathway substrate flux caused by changes to the test system relative to the control system such as the addition of compound being tested as a potential drug. |