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publicationDate 2004-04-08^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004067584-A1
titleOfInvention Defined systems for epithelial cell culture and use thereof
abstract The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.
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