| abstract |
The present invention relates to a chip-based device for three-dimensional microfluidic particle focusing and detection, characterized in which through the actions of fluidic driving force and dielectrophoretic forces, microparticles flow in the center of microchannels which enhances the accuracy of subsequent detection. The chip of the present invention is fabricated by first creating microchannels on a substrate for fluid flow, including specimen channels and sheath fluid channels, carrying out two-dimensional fluid focusing on particles in the sample flow, and fabricating microelectrodes in the microchannels to provide dielectrophoretic forces for three-dimensional focusing of particles. The present invention is applicable to the counting, determination, speed measuring and sorting of all kinds of microparticles, such as cells and blood cells. |