| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed5f33a87bf7838f235f298a57b270fc |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0073
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-13016 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate |
2007-03-28^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46e6b93711c02ff0c1790fe3eaf44560
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b06c487c4ed3411a9e8267429d05d70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f0a9528a78deaa8c5a9a14f5f7e696e |
| publicationDate |
2007-08-02^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2007178558-A1 |
| titleOfInvention |
Cloning, sequencing and expression of a Comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli |
| abstract |
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas ) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (M r , 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein. |
| priorityDate |
2000-07-18^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |