| abstract |
The invention is intended to provide a biochip that allows analysis to be simplified by eliminating a washing step, that is small and inexpensive, and that permits fast, reliable analysis, as well as a method of immunological analysis using such a biochip. The present invention is a biochip comprising a mixing chamber for mixing analyte and labeled antibody reacting therewith, a reaction chamber including fixing means to which anti-idiotype antibody has been fixed, and a detection part for detecting the analyte, wherein the labeled antibody is contained in the mixing chamber or an adjacent part, the labeled antibody comprises an antibody component consisting of an F(ab′) fragment or reduced IgG and a label bound at a predetermined binding ratio, the reaction chamber and the detection part are separated by a channel part through which the fixing means is not allowed to pass, and the anti-idiotype antibody is an anti-idiotype antibody against the labeled antibody and is a type of antibody that cannot bind to the reaction product of the analyte and labeled antibody. |