| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_946a15b69e8e7347c1f0be5eb5335f23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bf701ae42ffddc6396b8bca78245d5e1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a891a8afe10eca71af9f907e1460cbe0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_10357c401e5ab85cb42d6016f1a3c0b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8ebfc8d66ac1303ed1e3e999d65aa236 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-946 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8207
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8209 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C29-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11B1-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C35-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-12 |
| filingDate |
2011-07-22^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_361cbc1663d25d185b49cc94b35a089f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b21ed294e22416910519c987a80b20f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b03926041261085aee35063705498cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4f5fcdbf0a21a675ecbcaeca3550cae |
| publicationDate |
2012-02-09^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2012034653-A1 |
| titleOfInvention |
Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering |
| abstract |
Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum , allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019099407-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11549095-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110072998-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011195448-A1 |
| priorityDate |
2000-04-21^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |