| abstract |
This disclosure concerns a cytometry system including a handling system that presents single cells to at least one quantum cascade laser (QCL) source. The QCL laser source configured to deliver light to a cell within the cells in order to induce resonant mid-infrared absorption by one or more analytes of the cell. The system includes a laser source that excites at least one fluorophore present in the cell or on the surface of the cell and a fluorescence detector that measures the fluorescence intensity of the fluorophore. A mid-infrared detection facility detects the transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light and the fluorescence intensity are used to identify at least one cell characteristic. |