http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017009271-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_047b1b8eeba4a4c98b04bc510d01e293 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-90241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-26 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
| filingDate | 2014-12-18^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee72520ab1eab6b5ba82bd0231faf1e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa96376fe6c92637b92bf4e2a4f77880 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ebb5f9c5a554a9123cd5daa978ea56df |
| publicationDate | 2017-01-12^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2017009271-A1 |
| titleOfInvention | Screening Method |
| abstract | Provided is a high-throughput coupled enzyme method of screening for a tryptophan-2,3-dioxygenase (TDO) inhibitor compound and/or an indoleamine-2,3-dioxygenase (IDO) inhibitor compound, which method comprises: (a) reacting tryptophan with isolated IDO and/or isolated TDO in the presence of a test compound to form N-formylkynurenine; (b) reacting N-formylkynurenine from step (a) with isolated kynurenine formamidase to form kynurenine; and (c) detecting the kynurenine produced in step (b) and determining whether the test compound is a TDO and/or an IDO inhibitor compound or not from the presence or absence or quantity of the detected kynurenine, wherein step (a) is conducted in the presence of a reducing system suitable for converting IDO and/or TDO from the Fe 3+ to the Fe 2+ state, and which does not prevent the formation of kynurenine in step (c). |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111007173-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112557372-A |
| priorityDate | 2013-12-20^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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