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filingDate 2020-11-16^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-12-15^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022395834-A1
titleOfInvention Microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber
abstract A microfluidic device for and methods of using surface-attached posts and capture beads in a microfluidic chamber is disclosed. For example, the microfluidics device includes a pair of substrates separated by a gap and thereby forming a reaction (or assay) chamber therebetween. A field of actuatable surface-attached posts (e.g., magnetically responsive microposts) is provided on one or both of the substrates. The surface-attached posts are functionalized with capture beads. Additionally, methods are provided of functionalizing the surface-attached posts with the capture beads. Additionally, methods are provided of using the surface-attached posts that are functionalized with capture beads in a microfluidics device for binding a target of interest. Further, a bead spraying system and method is provided for spraying magnetically responsive and/or non-magnetically responsive beads atop and/or among a field of surface-attached microposts for use in a microfluidic device.
priorityDate 2019-11-15^^<http://www.w3.org/2001/XMLSchema#date>
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