| abstract |
Viruses and viral antigens harvested from embryonated eggs or tissue culture, whether acid-sensitive or acid-resistant, are purified and concentrated by processes which substantially eliminate the nonviral proteins present in the virus stock and thereafter permit the collection of a solute containing substantially all of the virus and viral antigens which were present in the starting stock. For both acid-resistant and acidsensitive viruses the processes of the invention comprise the elimination of nonviral protein by filtration or batch adsorption with resins discovered to selectively adsorb certain nonviral proteins. In the case of acid-sensitive viruses, the viruses are stabilized against subsequent acidification by the treatment of the resulting filtrate with a salt selected from the group consisting of ammonium sulfate, sodium sulfate, ammonium chloride and magnesium sulfate to complete the removal of nonviral protein. In both cases the viruses and viral antigens are acidified to pH 3-6 and adsorbed on preselected reactive materials from which the purified and concentrated virus is selectively extracted by elution with an eluent of pH 10-12 or higher which does not impair viral activity. |