| abstract |
Gels for electrophoresis such as polyacrylamide gels are formed in a neutral pH medium containing a salt solution of preselected concentration. This salt is incorporated in the gel and in subsequent use in electrophoresis is displaced upon application of an electrical potential (direct current) by ionic species which form a pH buffer in the gel. Prior to introduction of the buffer, the gel containing the neutral salt is storage stable for prolonged periods of time. The gel is reproducible since it is always formed under the same conditions and the buffer only thereafter introduced. |