| abstract |
A method is provided for synthesizing within a bacterial host, and secreting through the membrane of the host, a selected mature protein or polypeptide. The method involves: (a) cleaving a cloning vehicle to form a cleavage site after a promoter of either (1) a bacterial or phage gene within the cloning vehicle or (2) a DNA fragment of the bacterial or phage gene; (b) forming a hybrid gene by inserting into the cleavage site a non-bacterial DNA fragment which codes for precursor of the selected protein or polypeptide, including the signal sequence of the selected protein or polypeptide; (c) transforming the host with the cloning vehicle; and then (d) culturing the transformed host to secrete the selected protein or polypeptide. By this method, mature proteins or polypeptides can be produced, free of signal sequences or other chemical substituents, such as an f-met, on the proteins or polypeptides. |