| abstract |
A method of isolating blood-clotting Factor IX. A solution containing the Factor IX is adsorbed onto an adsorbent carrying α-hydroxylamine groups, and the factor is eluted. The Factor IX from the eluate is adsorbed onto a matrix carrying sulfated carbohydrates, with the conductivity adjusted to 13-17 mS/cm, and eluted with a salt gradient. The pure Factor IX is concentrated and freeze-dried. |