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filingDate 1991-02-27^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1992-12-22^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cfd0a947d0a09b4f6358a1ef014eebe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7da2519252cb282e310981c534b881aa
publicationDate 1992-12-22^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5173160-A
titleOfInvention Analysis of carrier ampholytes using immobilized ph gradients
abstract New synthesis and analysis procedures for novel crosslinked polyamines and ampholytes. Polyamines are crosslinked with tartaric acid ester, malonic acid ester, or polycarboxylic acid esters of the citric acid cycle. The resulting crosslinked polyamine may further react with other compounds such as an α, β-unsaturated or α-halo unsaturated carboxylic acids to prepare new ampholyte mixtures. The resulting novel ampholytes exhibit greater heterogeneity and complexity than presently prepared ampholytes, and can be used in analytical and preparative isoelectric focusing processes. n Novel ampholyte analysis process entails analyzing chemical compounds, usually ampholytes, used in isoelectric focusing processes. The ampholyte is isoelectrically focused on an immobilized pH gradient, and then immersed in a picric acid solution to c n GOVERNMENT OWNERSHIP n The funding for the subject matter described herein was partially provided by the U.S. Government under Grant Nos. NAS 9-17403 and NIH AI-20590. The U.S. Government may retain certain royalty-free rights in the invention claimed herein.
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