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filingDate 1995-04-11^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1996-09-03^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f6c08c2e245acb5ea5ec6771ffe43f0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f74f586728000b7e5bfcc906ea7032d
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publicationDate 1996-09-03^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-5552297-A
titleOfInvention Lead detection method and reggents utilizing aminolevulinic acid dehydratase and tertiary phosphines
abstract A method and kit for simplifying and improving the sensitivity and accuracy of a lead assay for a sample solution suspected of containing lead determines the extent of a reaction between a substrate and a disulfide enzyme in the presence of an activating reagent which contains a water-soluble tertiary phosphine reagent so as to increase the activity of the disulfide enzyme for reaction with the substrate. For a colorimetric determination of the enzyme activity a chromophore is formed upon reaction with a selected component of the sample solution in the presence of a colorimetric enhancing reagent. The colorimetric enhancing reagent contains a metal ion such as cupric ion or ferric ion which is soluble in the sample solution. The extent of the chromophore formation is then photometrically determined.
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