| abstract |
The object of the present invention is to provide a process for preparing a drug-containing liposome composition in which (1) a drug to be included into liposomes, particularly a physiologically active protein having a molecular weight of from 500 to 100,000, can be prevented from decomposition and (2) a high rate of drug inclusion can be attained; (3) the resulting liposome composition can be subcutaneously or intramuscularly administered and (4) makes contribution to sustained release of the drug. The process comprises (1) dissolving a lipid in a first organic solvent, (2) adding a drug-containing aqueous solution to the lipid solution, followed by emulsifying to obtain an emulsion, (3) mixing the emulsion at a low temperature with a second organic solvent in which the lipid is sparingly soluble, (4) collecting the precipitated fraction, and (5) suspending the precipitated fraction in an aqueous solvent. |