abstract |
A cell culture apparatus formed of a plurality of thin (e.g., 0.005'' or less), spaced, gas-permeable, silicone membranes sealed at their edges to form a bag-like vessel comprising one or more interior chambers suitable for containing cell culture media. A suitable portion of the membrane surfaces are of suitable thickness and surface area to provide structural integrity to the apparatus and sufficient gas permeability for cell growth within the chamber. |