| abstract |
A specific binding material labeled with an ultrafine inorganic phosphor 1 to 100 nm in particle size is disclosed The composition of the ultrafine inorganic phosphor is one of Ln 2 O 3 :Re, Ln 2 O 2 S:Re, ZnO, CaWO 4 , MO.xAl 2 O 3 :Eu, Zn 2 SiO 4 :Mn, and LaPO 4 :Ce,Tb, wherein Ln represents at least one element selected from La, Gd, Lu, and Y, Re represents at least one element selected from lanthanide elements, M represents at least one element selected from alkali earth metals, and x represents a value from 0.5 to 15. This ultrafine inorganic phosphor is prepared by one of the following processes a process of evaporation in a gas including an RF thermal plasma process, dc plasma thermal spraying, sputtering, glass crystallization, a sol-gel process, precipitation including hydrothermal synthesis, and a spraying process. An antibody which reacts specifically with an antigen to be measured is labeled with this ultrafine inorganic phosphor and reacted with a specimen, and the unreacted material is removed. The antibody bound to the specimen is detected by quantitatively observing fluorescence emitted by the phosphor. |