| abstract |
PCT No. PCT/SE96/01478 Sec. 371 Date Oct. 19, 1998 Sec. 102(e) Date Oct. 19, 1998 PCT Filed Nov. 14, 1996 PCT Pub. No. WO97/18472 PCT Pub. Date May 22, 1997A method of determining affinity and kinetic properties for the solution interaction between an analyte and a binding partner therefor, which method comprises: (a) mixing a solution of said analyte with a solution of said binding partner, contacting the resulting reaction solution with (i) immobilised binding partner, or analogue, for said analyte, and/or (ii) immobilised analyte, or analogue, and monitoring the binding of said analyte and/or binding partner in said reaction solution to the respective immobilised species to determine the variation with time of the concentration of free analyte and/or binding partner in said solution; and/or (b) contacting a solution of the reaction complex of said analyte and a binding partner therefor with (i) immobilised binding partner, or analogue, and/or (ii) immobilised analyte, or analogue, and monitoring the binding of said analyte and/or binding partner in said reaction complex solution to the respective immobilised species to determine the variation with time of the concentration of free analyte and/or binding partner resulting from dissociation of said reaction complex in said solution, and from said variation with time of free analyte and/or binding partner determining said affinity and/or kinetic properties. |