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titleOfInvention Anti-IgE antibodies and method of improving polypeptides
abstract The present invention relates to a method for adjusting the affinity of a polypeptide to a target molecule by a combination of steps, including: ( 1 ) the identification of aspartyl residues which are prone to isomerization; ( 2 ) the substitution of alternative residues and screening the resulting mutants for affinity against the target molecule. In a preferred embodiment, the method of substituting residues is affinity maturation with phage display (AMPD). In a further preferred embodiment the polypeptide is an antibody and the target molecule is an antigen. In a further preferred embodiment, the antibody is anti-IgE and the target molecule is IgE. In another embodiment, the invention relates to an anti-IgE antibody having improved affinity to IgE.
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