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filingDate 2000-11-17^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2004-01-06^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-6673540-B1
titleOfInvention Cell systems having specific interaction of peptide binding pairs
abstract This invention relates to modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; the fusion protein comprising a first peptide of a peptide binding pair, or segment of the first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, the fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.
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http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ2XNF5
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID136046180
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP04667
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ9YGV6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP33093
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP01328
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP68275
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP33092
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ01283
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID30546
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP01324
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP68274
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP33091
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ01282
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID136288194
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP01321
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID59109
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP68273
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP0CX81
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP33090
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP20392

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