| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5098eff6d67391cd857fae0aa3174bcb |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-38645 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S99-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11D3-386
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21C5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21H21-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D21B1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D06M16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12S11-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D06L4-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 |
| filingDate |
2007-07-30^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2011-09-13^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fdbeeef97c95f32a7206482267e1f3a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b00ebd86886969acb31ff6b5c71b5e5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ac0df0909f9b47061fbde10cef5b57d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a80882f258b00aae329d7d6230b8d2f3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7620bdfc6cc075398b3706814319026a |
| publicationDate |
2011-09-13^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8017372-B2 |
| titleOfInvention |
Cellulase variants |
| abstract |
The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:n a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9394092-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3354716-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9908675-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013158364-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012289450-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9951363-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10087401-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013138288-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10696460-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11104497-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11753222-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9850512-B2 |
| priorityDate |
1996-09-17^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |