http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8119353-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_85b959d2100bb308fcf773d125a37ab8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32ebcfed4a13158fb0704e2bcae27a65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd06fbbc53bdb9e91282f030f262f810 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_253f0e7c35c04b06088ca6f68a893697 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6846 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2008-04-03^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-02-21^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cf1860e61884bd42fca260eee9d1c9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_856a4ff45999d8c4e46731859f6ebdb4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30d5d3a3b346b1d5c31909635a6f6937 |
| publicationDate | 2012-02-21^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8119353-B2 |
| titleOfInvention | Rapid one-step reverse transcriptase PCR |
| abstract | The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C. |
| priorityDate | 2007-04-05^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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