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filingDate 2011-05-12^^<http://www.w3.org/2001/XMLSchema#date>
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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c43e52eb13b1257fd1ce924a205116b9
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publicationDate 2012-12-11^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8329466-B2
titleOfInvention Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
abstract The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. n The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.
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