http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8642263-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_108aa97bc2c4809a6c26cedf9451c71e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d92968c3123c8a92fabfa8d4e8a06c0b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78941ed5a01bfbcd48376e1fdb485b19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_68295ea218c99fd36838c41d5986925f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43b9bd6895347ffd96ce2a2f4132d7ad |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-02-04^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_864233bc8eaf15b1b73d50fb344b41cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f8c53a0a49a7474e3b6e631e1a52491 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76ffc431bbc02b9f12f12e5ee8f5baab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_003de2c4922592357b4dcbb5cc07b331 |
| publicationDate | 2014-02-04^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8642263-B2 |
| titleOfInvention | High throughput methods comprising analysis of repetitive element DNA methylation |
| abstract | Preferred aspects provide novel high-throughput, sensitive methods (e.g., real-time PCR-based (MethyLight™) reactions) for detection and/or measurement of global genomic 5-methylcytosine content, based on measurement of DNA methylation of Alu, LINE-1 repetitive sequences, and the chromosome 1 centromeric satellite alpha and juxtacentromeric satellite 2 repeat sequences. Additional aspects provide sensitive methods for determining the amount of a DNA (e.g., in formalin-fixed, paraffin-embedded tissues). Combined (mean) use of Alu and Sat2 repeat methylation measurements provides for a surprisingly close correlation with global genomic 5-methylcytosine content measurements obtained by HPLC. Methylation of Alu repeats was determined to be closely associated with HPLC-based global methylation levels, as was methylation of satellite 2 and LINE-1 global genomic 5-methylcytosine content. The assays provide surrogate markers for global genomic 5-methylcytosine content analyzes, and have substantial utility for high-throughput and population-based studies (e.g., genetic and dietary influences on global DNA methylation, folate deficiency, MTHFR gene polymorphisms, etc). |
| priorityDate | 2004-11-10^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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