| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_98da52b22570a3376a5571733ea234dc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a319a425a6f5d2fb80b99dddbc19a00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_40502008358c42b46c25ea918dbfd3c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c90d797d60834177e4b09eaccbcfd9e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5a5ae085853db5173d99daca7b6669
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3bcace8954ddba628f891d699a8c711d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-13 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5035
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate |
2009-07-30^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-04-07^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65a3aefbb6189c11aafb1cd2fd2cd7c0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5c7770de336296fe2d699ee56590992
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01af2cd1f449f64195efa2d4ec764f3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_013cebeb19553567134932dc3c8fa279
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60b49ac89a63140e7953cac0192f5e76 |
| publicationDate |
2015-04-07^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8999653-B2 |
| titleOfInvention |
Method for detecting membrane protein internalization |
| abstract |
The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10 −7 M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.75 V; or (3) an agent which binds specifically, by non-covalent bonding, with the fluorescent metal complex; (d) adding a metal ion which competes with the rare earth so as to form a non-fluorescent metal complex; (d) measuring the luminescence emitted by the reaction medium at the emission wavelength of the fluorescent metal complex and/or at the emission wavelength of the modulating compound when the compound is a fluorescent acceptor compound; and (e) comparing the signal measured in step d) with a reference signal measured on cells having been subjected only to steps a) and c). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11401321-B2 |
| priorityDate |
2008-07-31^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |