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filingDate 2011-01-31^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-08-25^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9115353-B2
titleOfInvention Method for nucleotide detection
abstract A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.
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