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titleOfInvention Glycerol 3- phosphate dehydrogenase for butanol production
abstract Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased K M for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.
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http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID3034794
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5364509
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226396047
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID1148
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID46937007
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226427686
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6305
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226400875
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID938
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID122283
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID439153

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