| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b4f4b32827fe99f9301ec6999b636ce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a16f72268f5a1eb4f08073323a474668 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-327 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-26004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
2013-10-28^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-03-21^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_015c9f4826a0b7d0f521f526898b4fc0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b411c5b21e351de1594f3783dbaacfb8 |
| publicationDate |
2017-03-21^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9598729-B2 |
| titleOfInvention |
Modified RNAse H and detection of nucleic acid amplification |
| abstract |
A reversibly modified ‘hot start’ RNase H enzyme composition is described for the improved CATACLEAVE™ probe detection of nucleic acid sequences in a test sample. A key feature of the enzyme composition is the ability to regulate the catalytic activity of the RNase H during the course of a reverse transcription-PCR cycle. Thus, RNase H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription. After cDNA synthesis is complete, RNase H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE™ probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNase H enzyme is amenable to high throughput applications requiring one step reverse transcriptase CATACLEAVE™ PCR in a single reaction mix. |
| priorityDate |
2010-05-25^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |