| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3c96af599b7d29b6715a68a0b4c224a1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y201-01037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate |
2013-02-21^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-03-28^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26d111a2e454e8fadb07e6528e644b55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_795e7ad2d68b270cfc098eb2c82b963a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abca5ea95ba68e846b189d2c0ed3fbb6 |
| publicationDate |
2017-03-28^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9605268-B2 |
| titleOfInvention |
Method for introducing an exogenous DNA by overcoming the restriction modification barrier of a target bacterium |
| abstract |
Disclosed is a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method includes the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A, 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule, and 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium. |
| priorityDate |
2012-03-23^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |