http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9617590-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b4ee13af463c18c765406625e1c5d44 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-414 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-4145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-414 |
filingDate | 2012-09-28^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-04-11^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac4f336ddcd50c731683df86e4c75133 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1c9423c22233986b05b2ae3e7839223 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_764172d85baf9fca44d00ae1cfd4ac41 |
publicationDate | 2017-04-11^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-9617590-B2 |
titleOfInvention | DNA sample preparation and sequencing |
abstract | This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10544455-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018119217-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10487357-B2 |
priorityDate | 2011-09-30^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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