http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9617590-B2

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filingDate 2012-09-28^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-04-11^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-04-11^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9617590-B2
titleOfInvention DNA sample preparation and sequencing
abstract This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.
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